产品货号：
GS2149
中文名称：
MspA1I内切酶
英文名称：
MspA1I
产品规格：
250U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：MspA1I内切酶，MspA1I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | CMG↑CKG |
| 来源 | Moraxella species A1 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 20 mM Tris-HCl(pH 7.6);300 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;10 mM MgCl2,200 μg/ml BSA,50%glycerol.|Store at -20℃.|For long term storage(more than 30 days),store at -70℃.? |
| Ligations | After 5-fold overdigestion with enzyme 60%of the DNA fragments can be ligated and recut.In the presence of 10%PEG ligation is better |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | NspBII |
相关搜索：MspA1I内切酶，MspA1I限制性内切酶